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When injections finished, all mice were euthanized, and the serum was harvested

Hober S, Ljung JL, Uhln M, Nilsson B (1999) Insulin-like growth factors I and II are unable to form and maintain their native disulfides under in vivo redox conditions

Immunoprecipitation (IP) and Co-immunoprecipitation (Co-IP) assay Cells or tissues were lysed in IP lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% Triton X-100, 0.5% NP-40, 1 mM EDTA, and 1 mM EGTA, 1 mM -glycerophosphate, 1 mM PMSF, and 1X PIC)

Int J Hyg Environ Health 254:114265 Tang R, Chen MJ, Ding GD, Chen XJ, Han XM, Zhou K, Chen LM, Xia YK, Tian Y, Wang XR (2013) Associations of prenatal exposure to phenols with birth outcomes

These biotypes are not independent conditions