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Extracellular flux assayglycolysis rate assay Cells were plated at a density of 7500 cells/well in XF96 micro-plates (Agilent Technologies) in DMEM/F12 supplemented with 10% FBS 48 h prior to the assay and cultivated in a humified incubator at 37 C with 5% CO 2

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While you do this, gradually apply pressure towards the ends of the cigarette

JMIR public health surveil: e8 Smith KC, Washington C, Welding K, Kroart L, Osho A, Cohen JE (2016) The cigarette stick as valuable communicative real estate: a content analysis of cigarettes from 14 low and middle-income countries

Among these proteins, HSP27 and phosphorylated HSP27 showed significantly elevated expressions among smokers