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GABA acts as an inhibitory neurotransmitter in vertebrates and is present across Bacteria, Archaea, and Eukaryota kingdoms, exhibiting a diverse range of roles in various organisms (Wu and Sun

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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

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It is well known that morphine can markedly lower GSH and cysteine levels in brain and peripheral tissues 109,110,111,112,113,114,115,116,117

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FASEB J 15:13351349 Sadrzadeh SM, Nanji AA, Price PL (1994) The oral iron chelator, 1,2 dimethyl3hydroxypyrid4one reduces hepatic free iron, lipid peroxidation and fat accumulation in chronically ethanolfed rats

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