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After 810 days growth, the cultures were shaken for 1 h (180 rpm, 37 C) to remove the microglia, then were shaken for 14 h (200 rpm, 37 C) to collect the OPCs

Research has shown that Nestorone, a progesterone analog, upregulates SIRT3 and restores the acetyl-superoxide dismutase 2 (Ac-SOD2)/superoxide dismutase 2 (SOD2) ratio by modulating the progesterone signaling pathway

2c, d) , and demonstrate increased peripheral glucose levels with stable body weights, echoing previous reports ( 13 )

2.6 Measures of reactive oxygen species (ROS) by the 2,7-dichlorofluorescein diacetate (DCFH-DA) assay 2,7-dichlorofluorescein diacetate (DCFH-DA) was used as probe for the estimation of ROS content in tissue homogenate