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After the solution had entered the column, 550 l of 100 mM HEPES p H 7.0 were loaded on the column and 300 l of 100 mM HEPES p H 7.0 were used to elute the protein

Shaw AT, Winslow MM, Magendantz M, Ouyang C, Dowdle J, Subramanian A et al

In particular, these diverse metabolic control mechanisms provide a resilient or flexible adaptation to stress from food insecurity as well as overeating 5