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1 Introduction According to the most recent statistics published in World Drug Report, in 2021, approximately 36.3 million people (ranging from 19.6 to 53 million) are estimated to suffer from drug or substance use disorders (SUDs) all over the world ( Indeed, the etiology of SUDs and addiction is complex and modulated by a wide variety of factors, ranging from individual genetic make-up and exposure to environmental and emotional stress to personality and environmental factors like socioeconomic status and having access to drugs ( Regardless of the type of the substance of choice, all addictive substances are common in driving reward systems of brain through raising dopamine levels and altering brains function ( The heritability of SUDs ranges from 39-72% ( Methamphetamine and opiate addiction also comprise considerable percent of SUDs in Iran ( From biological standpoint, Methamphetamine (METH) exposure alters dopaminergic ( The mechanism of action of morphine, as an agonist for G-protein coupled opioid receptors, almost involves binding to those receptors, induction of receptor conformational changes ( Both METH and morphine which are known to introduce cellular reduction and oxidation (redox) imbalance are potentially able to negatively affect protein post-translational modifications (PTMs) which are sensitive to redox homeostasis ( a cysteine residues ( 2 Materials and methods 2.1 Participants A total of 160 blood samples were randomly selected from 50 opium users alive and 30 amphetamine user that were from Tehran Forensic Toxicology Laboratory and 80 healthy people as a control group