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Genetic polymorphisms affecting VEGF expression and activity also influence its role in DR

Primary antibodies used were: mouse monoclonal (IgG2A) anti-PSD-95 clone K28/43 (1:200, Neuromab, UC Davis, Davis, CA), mouse monoclonal IgG1 anti-PSD-95 clone 7E3-1B8 (1:250, Thermo Fisher Scientific, Waltham, MA, cat#: MA1-046) anti-guinea pig polyclonal anti-Bassoon (1:300, Synaptic Systems, Gottingen, Germany, cat# 141 004), rabbit polyclonal anti-Bassoon (1:300, Synaptic Systems, cat #: 141 003), chicken anti-GFP (1:2000, Abcam, Cambridge, MA, cat# ab13970), rabbit monoclonal anti-GluN1 (1:500, AB9864, Millipore Sigma), mouse monoclonal (IgG2A) anti-GluN2A clone N327/95 (1:250, Neuromab), mouse monoclonal (IgG2B) anti-GluN2B clone N59/36 (1:250, Neuromab), mouse monoclonal (IgG1) anti-GluA1 clone N355/11 (1:250, Neuromab), rabbit polyclonal anti-GluA2(1:500, Synaptic Systems, cat#: 182 103), mouse monoclonal (IgG2A) anti-Synaptotagmin 1 (1:500, Synaptic Systems, cat#: 105 011)

Text by Godfre Leung, Darsie Alexander, Maria Jose Herrera, Claudia Calirman, Erica Battle, Dvid Fehr, Luigia Lonardelli, Hiroko Ikegami, Ed Halter, Charlotte Cotton, Martin Harrison, Tom Pospiszyl

PMID 7844111

4.4 Nanostructured lipid carriers NLCs are advanced lipid-based systems developed by incorporating both solid and liquid lipids (up to 30%), resulting in enhanced drug encapsulation, stability, and skin permeation