Beads were washed three times with PBS and 5 l of bound proteins were eluted by adding 2 Laemmli buffer and incubating at 100 C for 5 min
You must weigh the risk of side effects against the value of any medication
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Bacterial isolation and taxonomic identification Root samples collected from the Ca800 treatment were used for bacterial isolation, as the Ca-enriched taxa (ASVs) were identical to those found in the Ca300 and Ca400 treatments, but their relative abundances were highest in the Ca800 treatment

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