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Following extraction, the samples were centrifuged, and the supernatant was passed through a mixed-bed Dowex column containing 300 L each of Dowex H + and Dowex Ac (100200 mesh) ( 2 SO 4 ), following the protocol outlined by Yaghoubi et al ( 2.8 Determination of the overall antioxidants Total antioxidant capacity in the shoot samples was measured using ferric reducing/antioxidant power (FRAP) reagent, containing 0.3 M acetate buffer (pH 3.6), TPTZ (0.01) and FeCl 3 (0.02 M), followed by reading the OD at 600 nm ( 2 O 2 decomposition at 240 nm ( 2.9 Determination of anthocyanins, phenolics and flavonoids To measure the anthocyanins content, methanol: HCl (99:1 v/v) was used for homogenizing the samples, followed by incubating the extracted samples at 25C for 24 h in the dark conditions and centrifuging at 4000 g for 5 min