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In addition to oxidative stress, excessive copper ion accumulation alters energy metabolism patterns within cardiomyocytes by inducing abnormal aggregation of thioctylated proteins and loss of iron-sulfur cluster proteins in the respiratory chain complex through direct binding to thioctylated proteins in the mitochondrial TCA cycle (76)

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The role for, current use, and yield of biochemical testing for durable alcohol metabolites, e.g., phosphatidylethanol (PETH) remains uncertain

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doi: 10.1111/j.1471-4159.1974.tb04379.x