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Following overnight incubation, cells were detached, resuspended in phenol red-free DMEM (Thermo Fisher Scientific, 21063-029), and seeded into white 96-well plates with clear bottoms (Greiner Bio-One, Courtaboeuf, France) at a density of 40,000 cells/well (200 L/well of a 2 10 5 cells/mL suspension)

The molecule responsible turned out to be GHK-Cu

Cells were then stained with hematoxylin (DAKO, Mayers Hematoxylin, S3309) for 30 s, washed in ammonia for 30 s and then washed in dH 2 O

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