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The two main subtypes, classified by morphology and region, are protoplasmic and fibrous astrocytes

[DOI] [PubMed] [Google Scholar] 10.Apeland T, Mansoor MA, Pentieva K, McNulty H, Strandjord RE

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]