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Samples were then serially diluted with phosphate buffer to achieve an approximate range of 30 to 300 colonies on the plate

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Following two rounds of 2D classification, 208,094 and 152,620 particles were selected for ab-initio reconstruction, respectively

5.3 Advantages of intranasal delivery Intranasal (IN) delivery offers several advantages over other common routes, such as oral administration, particularly in enhancing therapeutic efficacy and patient compliance