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doi: 10.1016/S0300-483X(00)00231-6 52 DworskiRMurrayJJRobertsLJOatesJAMorrowJDFisherLet al

All animal experiments were approved by the Animal Ethics Committee of Qiqihar Medical University (Approval No.: 2022401)

Lifestyle choices, including exercise, diet, and oral health, are critical in influencing the aging process

Then, cells were centrifuged and resuspended into 2 mL of prewarmed hypotonic solution (KCl 0.075 mmol L 1 ) and incubated in 14 mL of this solution for 15 min at 37 C