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For the observation of mitochondria generating ROS, the cells were incubated with HBSS (Sigma-Aldrich) containing 5 M MitoSOX for 10 min at 37 C in the dark and were then washed with PBS 3 times to remove any residual dye

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It's important to note that some foods marketed as B12 sources, such as spirulina, nori and other algae, contain B12 analogues that are not reliably bioactive in humans and should not be relied upon as primary B12 sources